只需添加水:一种新的性封闭试剂,用于轻松净化在固态阶段合成的
Vittorio Montanari1, Krishna Kumar
1Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Journal of the American Chemical Society
|August 5, 2004
概括
一种新的性封闭试剂在固相合成 (SPPS) 过程中标记未反应的氨基. 这种方法简化了净化,通过色染色学去除删除产物以获得纯产量.
科学领域:
- 有机化学 有机化学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 固相合成 (SPPS) 对于生产来说至关重要.
- 从删除序列中净化全长的过程可能具有挑战性.
- 需要有效的上限策略来提高SPPS产量和纯度.
研究的目的:
- 为增强的SPPS引入一种新的化封闭试剂.
- 为了改善通过SPPS合成的的净化.
- 开发一种有效去除删除产品的方法.
主要方法:
- 一种新的三价盐 (试剂1) 被合成和特征.
- 试剂1用于标记在SPPS中合后未发生反应的氨基.
- 光标记使删除序列使用闪光光染色学.
主要成果:
- 化封闭试剂有效标记了未反应的氨基.
- 在标准的SPPS条件下,甲基胺是稳定的.
- 闪光色谱成功地将标记的删除产品从所需的中分离出来.
结论:
- 新型化封装试剂促进了SPPS中的净化.
- 这种方法提供了一个强大的策略,以获得纯粹的,全长的.
- 开发的技术为酸净化挑战提供了有效的替代方案.
相关概念视频
Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
Testing Water Quality
When the quality of water for concrete preparation is uncertain, its impact on the setting time of cement and compressive strength of mortar is assessed by comparison with de-ionized or distilled water benchmarks. American Society for Testing and Materials (ASTM) C1602 requires the setting times to be within 90 minutes of the control, British Standard (BS) 3146:1980 allows a 30-minute variance in the initial setting, while British Standards European Norm (BS EN) 1008 specifies initial setting...
Curing Methods
Concrete members with a small surface-to-volume ratio are cured by oiling and moistening the forms before casting the concrete member. These forms can be left in place for a prolonged period to prevent moisture loss, and can be wetted if made of a material suitable for wetting. If the forms are removed early, the concrete member is moistened and covered with polythene sheets to maintain moisture. For large horizontal concrete surfaces exposed to dry weather, a temporary covering is suspended...
Plasticizers
Water-reducers, or plasticizers, are chemical admixtures used in concrete to improve strength and workability. These additives reduce the water-cement ratio without compromising workability, lower the cement content while maintaining the same workability, or increase workability to assist concrete placement in inaccessible areas.
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
Superplasticizers
Superplasticizers are advanced admixtures that enhance the workability of concrete by lowering the water content without compromising the strength of the material. These substances are highly effective water reducers, improving concrete flow, making it easier to work with, and enabling concrete to reach inaccessible areas or densely reinforced sections without mechanical vibration. The key components in superplasticizers are either sulfonated melamine or naphthalene formaldehyde condensates,...
Waterproofing and Anti-Bacterial Admixtures in Concrete
Concrete's susceptibility to water absorption is due to the capillary action within the pores of its hydrated cement paste. This action draws water in, creating the need for waterproofing admixtures to prevent such penetration. The efficacy of these admixtures is contingent upon the water pressure, with variations arising from different conditions such as rain, capillary rise, or hydrostatic pressure in structures intended to hold water.
Waterproofing admixtures render concrete hydrophobic,...
Waterproofing admixtures render concrete hydrophobic,...


