在C. elegans中通过一种新型E3/E4-多双化复合体调节肌导向的护卫体UNC-45
Thorsten Hoppe1, Giuseppe Cassata, José M Barral
1Department of Molecular Neurogenetics, Adolph-Butenandt-Institute, Ludwig Maximilians University, Schillerstrasse 44, 80336 Munich, Germany.
Cell
|August 6, 2004
概括
研究人员发现了一种新的E3/E4复合物,CHN-1/UFD-2,该复合物多元化UNC-45. 这种复合物对C. elegans的肌肉组织和肌肉功能至关重要,影响细胞结构.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 肌组织成细胞结构需要严格的时间和空间调节.
- 在C. elegans中,UNC-45在厚丝线组装过程中充当肌素的陪伴剂和Hsp90辅伴剂.
- 在unc-45的突变导致C. elegans的和无组织的肌纤维细胞.
研究的目的:
- 为了确定负责UNC-45规则的分子机器.
- 调查CHN-1和UFD-2在UNC-45无处不在中的作用.
- 阐明肌肉组合中CHN-1/UFD-2复合体和UNC-45之间的功能关系.
主要方法:
- 采用CHN-1和UFD-2进行体外多双化试验.
- 对unc-45温度敏感突变体的分析.
- 研究C. elegans中chn-1功能丧失和UNC-45过度表达的影响.
主要成果:
- 一个新的E3/E4复合体,CHN-1 (C. elegans CHIP ortholog) 和UFD-2被确定.
- 这种复合物被发现是必要的,并且足以在体外进行UNC-45的多重双化.
- 丧失chn-1部分抑制了unc-45突变表型,而unc-45过度表达在chn-1缺乏的虫中导致了严重的肌肉失调.
结论:
- CHN-1和UFD-2形成了一个功能性的E3/E4复合体.
- UNC-45是CHN-1/UFD-2无处不在复合物的生理相关基质.
- 这个复合体在调节UNC-45的功能中起着至关重要的作用,以确保正确的肌肉组装和运动.
相关概念视频
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
The Spindle Assembly Checkpoint
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Directing Proteins to the Rough Endoplasmic Reticulum
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Regulation of the Unfolded Protein Response
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...


