层状流动激活了血管内皮细胞中的过氧体增殖器激活受体-玛
Yi Liu1, Yi Zhu, Francois Rannou
1Division of Biomedical Sciences, University of California at Riverside, Riverside, CA 92521-0121, USA.
Circulation
|August 18, 2004
概括
稳定的层状流动激活了内皮细胞中的过氧体增殖器激活受体- (PPARgamma). 这一过程依赖于脂酶A2和细胞染色体P450,通过产生PPARgamma配体产生抗炎作用.
科学领域:
- 心血管生物学 心血管生物学
- 内皮细胞功能 内皮细胞功能
- 炎症的分子机制
背景情况:
- 稳定的层状流体表现出其他保护性质.
- 层状流对血管内皮细胞 (ECs) 产生抗炎作用.
研究的目的:
- 为了研究在层状流下在EC中氧酶增殖器激活受体- (PPARgamma) 的激活.
- 为了阐明与这种激活相关的抗炎作用.
主要方法:
- 使用培养EC的流通道来研究PPARgamma激活.
- 分析了PPAR响应元素 (PPRE) 活性和CD36 mRNA表达.
- 使用GAL-PPARgamma-LBD融合蛋白和制药抑制剂 (PLA2,CYP450s) 调查了依赖联体.
主要成果:
- 层状流激活了PPRE活性,并在EC中增加了CD36 mRNA.
- 通过流动激活PPARgamma是依赖于连接体的,需要PPRE.
- 抑制PLA2和CYP450的药物阻断了流动诱导的PPARgamma激活.
- 来自流媒体的脂质提取物在其他细胞类型中激活了PPARgamma.
- 帕克林激活抑制了细胞因子诱导的核因子-kappaB激活和ICAM-1表达.
结论:
- 层状流动以依赖于连接体的方式激活EC中的内源PPARgamma.
- 脂酶A2 (PLA2) - 细胞染色体P450 (CYP450) 途径介导流动诱导的PPARgamma配体的产生.
- 这些PPARgamma配体在多种细胞类型中发挥抗炎作用.
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