叶酸介导的细胞向和细胞毒性使用热敏微凝
Satish Nayak1, Hsienming Lee, Jean Chmielewski
1School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
Journal of the American Chemical Society
|August 19, 2004
概括
叶酸功能化的微凝有效地准癌细胞并逃脱内分泌体. 温度触发的粒子崩会诱导细胞毒性,这表明这些聚合物纳米粒子具有潜在的抗瘤活性.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 细胞生物学 细胞生物学
背景情况:
- 针对性地向癌细胞输送治疗剂仍然是一个重大挑战.
- 聚合物纳米粒子为药物输送和癌症治疗提供了潜力.
- 叶酸受体介导的内细胞结核是癌细胞向的一个有前途的途径.
研究的目的:
- 设计和评估光,热敏的微凝与叶酸功能化,用于向癌细胞的输送.
- 研究这些纳米粒子的细胞吸收机制和细胞内命运.
- 评估温度触发的粒子聚合对诱导癌细胞死亡的潜力.
主要方法:
- 光,热敏的微凝的合成.
- 用叶酸进行表面功能化.
- 在无叶酸介质中与KB癌细胞进行化.
- 对焦显微镜和流细胞计用于细胞吸收分析.
- Lysotracker 红色染色以追踪内体细胞逃逸.
- 温度诱导的粒子崩和细胞毒性测定.
主要成果:
- 通过 KB 细胞对叶酸修饰的微凝进行有效的受体介导的内细胞分解.
- 叶酸改性颗粒的吸收率高于阴离子对照组.
- 有证据表明颗粒物从内体逃到细胞质中.
- 取决于温度的粒子膨胀和崩.
- 通过触发的粒子聚合诱导显著的细胞毒性.
结论:
- 开发的聚合物纳米粒子通过叶酸受体介导的内细胞分裂有效地向癌细胞.
- 证明了纳米颗粒的内体逃逸和细胞质输送.
- 利用热反应性质触发粒子聚合并诱导显著的癌细胞死亡.
- 这些纳米粒子显示出开发具有潜在抗瘤活性的新型癌症疗法的前景.
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