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基于扩张的时间Shh梯度在指定脊椎动物数字身份的证据

Brian D Harfe¹, Paul J Scherz, Sahar Nissim

¹Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, 1600 SW Archer Road, Gainesville, FL 32610, USA.

|August 19, 2004

概括

极化活动的区域产生Sonic Hedgehog (Shh),对肢体发育至关重要. 产生SHH的细胞形成后部数字,暴露时间,而不仅仅是度,塑造数字身份.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

后肢芽中的极化活动区 (ZPA) 对于肢体发育至关重要.
由ZPA产生的Sonic Hedgehog (Shh) 蛋白质是调节前后模式的关键形态原体.
之前的模型专注于 Shh 度梯度用于数字规范.

研究的目的:

为了确定肢体中SHH产生细胞的发育命运.
阐明Shh在前后轴上指定数字标识中的作用.
改进对肢体发育中的SHh形态原活性的理解.

主要方法:

在小鼠模型中利用基于复合酶的血统追踪.
基于SHH生产,分析了细胞对肢体数字的贡献.
在成长中的肢体内对SHH可访问性的进行了遗传研究.

主要成果:

产生SHH细胞的后代占据了最后面的两个数字,并为中间数字做出了贡献.
数字的规格取决于Shh度 (前数字) 和暴露时间 (后数字).
对Shh可访问性的基因操纵支持一个时间和空间梯度效应模型.

结论:

产生SHH的细胞对后 digit 的形成有着显著的贡献.
肢体数字的身份是由Shh度和时间暴露的组合决定的.
双梯度模型 (空间和时间) 最好解释 Shh 形态基因在四肢模式中的活性.

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