激活T细胞的核因子含有Fos和Jun
J Jain1, P G McCaffrey, V E Valge-Archer
1Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Nature
|April 30, 1992
概括
含有Fos和Jun蛋白质的转录因子AP-1是NF-AT的新合成的核成分. 这一发现揭示了T细胞中NF-AT的两阶段激活过程.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞信号传输 细胞信号传输
背景情况:
- 激活T细胞的核因子 (NF-AT) 对于T细胞中介素-2 (IL-2) 基因诱导至关重要.
- NF-AT是免疫抑制药物如环素A (CsA) 和FK506.6的关键标.
- NF-AT诱导涉及CsA敏感和CsA抗性的激活步骤.
研究的目的:
- 为了识别NF-AT.的新合成的核成分.
- 阐明在T细胞中NF-AT激活背后的分子机制.
- 描述NF-AT组件之间的相互作用.
主要方法:
- 电泳运动转移试验 (EMSAs) 用于研究蛋白质-DNA结合.
- 西部涂抹检测特定的蛋白质 (Fos,Jun).
- 复制和共传染实验以验证功能相互作用.
主要成果:
- NF-AT的可诱导核形式包括Fos和Jun蛋白,识别AP-1作为合成组件.
- 在未受刺激的T细胞中确定了先前存在的NF-AT结合因子.
- NF-AT激活涉及CsA敏感修改/转移一个先前存在的因子,其次是CsA不敏感的AP-1 (Fos/Jun) 的添加.
结论:
- 转录因子AP-1是NF-AT的新合成成分.
- NF-AT激活是一个多步骤的过程,涉及既有因素和新合成的因素.
- 这一发现为T细胞激活和免疫抑制目标提供了更深入的理解.
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