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通过d(GGGT) 形成一个相互锁定的四重复二元体
Yamuna Krishnan-Ghosh1, Dongsheng Liu, Shankar Balasubramanian
1Contribution from the University Chemical Laboratories, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.
Journal of the American Chemical Society
|September 2, 2004
概括
四核酸d ((GGGT) 自组合成平行四重复二次体. 确定了两种不同的物种,四合体和八合体,揭示了新的结构安排,包括带有独特GTGT四合体的滑动四合体二合体.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- DNA 序列可以自组装成复杂的结构.
- G四重复体是具有多种生物作用的非正规DNA结构.
- 已知d ((GGGT) 序列形成四重复结构.
研究的目的:
- 为了研究四核酸的自我组装d(GGGT).
- 为了描述由此产生的四重复物种的结构.
- 为了阐明四重复二分体形成的机制.
主要方法:
- 紫外线化研究以识别不同的物种及其热稳定性.
- 循环二重化 (CD) 光谱法以确定四重复拓.
- 负离子纳米电子喷雾电离质谱法 (纳米-ESI-MS) 来确定复杂的固体测量.
- 高分辨率的 (1) H核磁共振 (NMR) 光谱法,以解决原子层结构.
主要成果:
- 确定了两种不同的四重体物种,Q (l) (四重体,d) (GGGT) (4)) 和Q (h) (八重体,d) (GGGT) (8).
- 这两种物种都表现出平行四重复体的特征CD光谱.
- 高分辨率的NMR证实了d(GGGT) ((4) 作为一个C(4) 对称的平行四分子四重复.
- d(GGGT) ((8) 结构涉及"滑动"平行四重复的二元化,形成一个额外的G-四边形和一个新的GTGT四边形在3'端.
结论:
- 这个d(GGGT) 序列形成了稳定的平行四重复数及其二次数.
- 发现了一种新的"滑动"四重复二元结构,涉及分子间G-四边形形成.
- 这项研究揭示了G-quadruplex结构多样性和自我组装机制的新见解.
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