一个真核生物基因组的转录调节代码
Christopher T Harbison1, D Benjamin Gordon, Tong Ihn Lee
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.
Nature
|September 3, 2004
概括
科学家通过识别由调节器结合的保存DNA序列来绘制酵母的转录调节代码. 这揭示了环境条件如何影响基因调节,预测调节器的功能.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 系统生物学 系统生物学
背景情况:
- 结合DNA的转录调节器通过结合特定的DNA序列来控制基因表达.
- 比较基因组学有助于识别酵母中保存的cis-regulatory序列.
- 单纯的基因保护并不能表明调节器的结合或定时.
研究的目的:
- 为了绘制酵母的转录调节代码.
- 在各种条件下识别由调节器约束的序列元素.
- 了解监管要素的组织和环境依赖性使用.
主要方法:
- 利用比较基因组学来识别Saccharomyces物种中保存的DNA序列.
- 集成序列保存与实验数据的调节器结合在不同的条件.
- 分析了促进区域内的监管要素的组织.
主要成果:
- 构建了酵母的转录调节代码的初始地图.
- 确定特定的DNA序列元素,由调节器结合.
- 证明环境特定的监管者绑定预测了许多监管机构的功能模型.
结论:
- 这项研究提供了酵母转录调节的基本地图.
- 监管元素的环境依赖结合对于理解基因表达动态至关重要.
- 这种方法可以根据酵母的结合模式预测许多酵母转录调节者的功能.
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