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相关概念视频

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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相关实验视频

Updated: Jun 10, 2026

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12:17

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Published on: December 2, 2010

在T细胞受体CD3epsilon链中的内质网膜保留信号.

A Mallabiabarrena1, M Fresno, B Alarcón

  • 1Centro de Biología Molecular, Universidad Autónoma de Madrid, CSIC, Spain.

Nature
|June 18, 1992
PubMed
概括
此摘要是机器生成的。

这是T细胞受体复合体.

更多相关视频

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

相关实验视频

Last Updated: Jun 10, 2026

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12:17

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Published on: December 2, 2010

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • T细胞受体 (TCR) 复杂子单元经常被降解或保留在内质网膜 (ER) 中.
  • 多子单元TCR复合体的正确组装对于其细胞表面表达至关重要.
  • 了解ER保留机制是控制蛋白质贩运的关键.

研究的目的:

  • 为了确定负责ER保留的CD3epsilon子单元内的特定氨基酸序列.
  • 阐明这些序列在调节TCR复合体组合和细胞表面表达中的作用.

主要方法:

  • 在人类CD3epsilon子单元的细胞质尾部内进行了一系列的删除工程.
  • 评估修改的CD3epsilon链的细胞表面表达.
  • 将已识别的保留序列添加到CD4中,以独立测试其功能.
  • 在关键的10氨基酸序列上进行了位点定向的突变发生.

主要成果:

  • 在CD3epsilon细胞尾中删除171-180的氨基酸使得单独链的细胞表面表达成为可能.
  • 这种10氨基酸序列在与CD4融合时赋予了ER保留.
  • 突变检测显示,这种序列中的氨酸和氨酸残留物对ER保留至关重要.

结论:

  • 在CD3epsilon细胞尾中的特定的10氨基酸序列作为ER保留信号.
  • 这种信号必须在TCR复合组装过程中被掩盖,以获得适当的细胞表面表达.
  • 这些发现提供了关于蛋白质质量控制和在ER中的贩运的见解.