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相关概念视频

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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相关实验视频

Updated: Jul 9, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

通过液晶NMR光谱学评估一个小蛋白质的骨干质子位置和动态.

Tobias S Ulmer1, Benjamin E Ramirez, Frank Delaglio

  • 1Contribution from the Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Journal of the American Chemical Society
|September 17, 2004
PubMed
概括

核磁共振 (NMR) 二极合器完善了蛋白质G B3域结构. 这项研究探讨了键平面性和N-H向量方向,提高了结构精度.

科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学

背景情况:

  • 蛋白G (GB3) 结构的第三个IgG结合域之前通过X射线结晶学确定.
  • 核磁共振 (NMR) 是一种用于探测分子结构和动态的强大技术.
  • 液晶介质或凝中的蛋白质对齐对于测量二极合非常重要.

研究的目的:

  • 通过使用NMR测量脊柱双极合来完善GB3结构.
  • 为了研究键平面性和N-H向量的方向.
  • 评估基于二极合数据的结构模型的准确性.

主要方法:

  • 使用NMR收集了广泛的蛋白质骨干单键二极合.
  • 使用了各种调整介质,包括双,聚乙烯甘醇,细丝菌体和充电的烯胺凝.
  • 精制了 GB3 晶体结构与测定的 (13) C(alpha) - ((13) C' 和 (13) C'- ((15) N 双极合器相对应.

主要成果:

  • 精细化改进了实验和预测的 (15) N-(1) H(N) 和 (13) C(alpha) - ((1) H(alpha) 双极合之间的一致性.
  • 观察到键扭转角度欧米茄偏差与N-H向量的方向之间存在弱的反对应关系.

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Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
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NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
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NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

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Last Updated: Jul 9, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
09:25

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Published on: November 1, 2024

  • 发现GB3骨干的胺序列参数 (S) 是高度均的 (+/-7%).

    • 结论:

    • 核磁共振二极合为提炼蛋白质结构提供了有价值的约束.
    • 这项研究为结构建模中的C(alpha) -C'-N-H扭转角提供了更准确的近似.
    • GB3表现出同质的骨干动力学,在N-H和C(alpha) -H矢量方向上有轻微的偏差.