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相关概念视频

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

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相关实验视频

Updated: May 11, 2026

Isolation of Murine Valve Endothelial Cells
09:34

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Published on: August 21, 2014

血管内皮生长因子受体-2或Tie-2在周围血液细胞上的表达定义了能够重新内皮化功能性细胞群.

Grzegorz Nowak1, Azza Karrar, Carolina Holmén

  • 1Division of Transplantation Surgery, Karolinska University Hospital-Huddinge, Karolinska Institute, Stockholm. Sweden.

Circulation
|September 24, 2004
PubMed
概括

表达VEGFR-2或Tie-2受体的周围血液细胞可以分化为内皮细胞并促进血管修复. 这些发现支持基于细胞的血管疾病治疗方法.

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相关实验视频

Last Updated: May 11, 2026

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Published on: August 21, 2014

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科学领域:

  • 心血管生物学 心血管生物学
  • 干细胞研究 干细胞研究
  • 血管新生的产生.

背景情况:

  • 像VEGFR-1,VEGFR-2和Tie-2这样的受体氨酸激酶对心血管发育和血管生成至关重要.
  • 了解表达这些受体的外周血细胞是血管损伤治疗应用的关键.

研究的目的:

  • 描述表型和表达VEGFR-1,VEGFR-2和Tie-2的周围血液细胞的功能.
  • 在血管损伤模型中评估它们的治疗潜力.

主要方法:

  • 流细胞测量用于识别周围血液单核细胞中的VEGFR-1+,VEGFR-2+和Tie-2+细胞.
  • 试验室差异化测试以评估内皮和光滑肌肉细胞潜力.
  • 在体内研究使用气球受伤的小鼠大腿动脉来评估内皮质化能力.

主要成果:

  • 在单核细胞中,VEGFR-1+,VEGFR-2+和Tie-2+细胞代表着不同的群体,主要表达单细胞/巨细胞标记物.
  • 只有VEGFR-2+和Tie-2+细胞在体外分化为内皮细胞,并在体内有助于重新内皮细胞化.
  • 这些细胞也分化为光滑肌肉细胞,并在体内繁殖.

结论:

  • 表达VEGFR-2和/或Tie-2的外周血液细胞在功能上有能力进行再内皮质化,并且在体内具有增殖能力.
  • 这些发现表明血管疾病的潜在基于细胞的治疗策略.