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Updated: Jul 18, 2026

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
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突变对RNA迪尔斯-阿尔德拉酶的影响

Theodore M Tarasow1, Elizabeth Kellogg, Benjamin L Holley

  • 1Department of Chemistry, College of Physical and Mathematical Sciences, North Carolina State University, Raleigh, North Carolina 27695-8204, USA.

Journal of the American Chemical Society
|September 24, 2004
PubMed
概括

工程RNADiels-Alderases (DA22) 通过突变发生和体外选择表现出增强的催化效率. 这些改进的酶保持了特异性,其中一种变体显示出新的金属依赖性.

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