一氧化碳从肌球蛋白逃离到生理温度下溶液中的过程
Yasutaka Nishihara1, Masaaki Sakakura, Yoshifumi Kimura
1Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.
Journal of the American Chemical Society
|September 24, 2004
概括
一氧化碳 (CO) 通过特定的部位逃离髓质蛋白,受 (Xe) 的影响. Xe 压力影响 CO 逃逸率,揭示了在释放到溶液之前在 Xe (1) 位点的 CO 捕获.
科学领域:
- 生物物理学的生物物理.
- 物理化学 物理化学
背景情况:
- 肌球蛋白 (Mb) 是一种能结合和储存氧气的蛋白质.
- 一氧化碳 (CO) 是一种连接物,它与Mb.中的血红素铁结合.
- 了解连接体逃脱机制对于蛋白质功能研究至关重要.
研究的目的:
- 为了研究一氧化碳 (CO) 连接体从肌球蛋白 (Mb) 的逃生途径.
- 阐明 (Xe) 在调节二氧化碳逃逸动态中的作用.
- 为了确定涉及带释放的特定对接点.
主要方法:
- 使用过渡格 (TG) 技术来测量分子体积变化.
- 研究是在生理温度下进行的.
- 应用了不同的异压力来观察它们对二氧化碳逃逸率的影响.
主要成果:
- 显而易见的CO逃逸率和体积收缩随着异压力而增加.
- 压力依赖与Xe(1) 地点的异数量相关.
- 发现二氧化碳在逃到溶剂之前被困在Xe (1) 位点.
结论:
- A Xe(1) 捕获地点影响了Mb.的二氧化碳排放.
- 光激发释放CO,而不是被困的XE,从XE被困的MbCO.
- 存在两种不同的CO逃生途径,一种是主导性和Xe1) 依赖性,另一种是当Xe阻止Xe1) 站点时使用的.
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