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Updated: Jul 19, 2026
13:51
The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
酵母基因 (BEM1) 对于细胞两极化是必要的,其产物含有两个SH3域
J Chenevert1, K Corrado, A Bender
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Nature
|March 5, 1992
概括
研究人员在Saccharomyces cerevisiae中确定了特定的BEM1基因突变,这些突变会在交配期间破坏细胞两极分化. 这些bem1-s突变突出了BEM1的特征.
科学领域:
- 细胞生物学 细胞生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 细胞极化对于细胞功能至关重要,涉及建立细胞轴和细胞骨组织.
- 连接细胞骨与选择的细胞轴或部位的精确机制仍然不完全理解.
- 在植物生长和交配期间,Saccharomyces cerevisiae表现出细胞两极分化,对不同的细胞内和细胞外信号作出反应.
研究的目的:
- 为了研究细胞极化对细胞外交配费洛蒙的反应的分子基础.
- 在酵母中识别和表征激素诱导的细胞极化所必需的基因.
主要方法:
- 酵母突变的分离和特征,特别缺陷交配费洛蒙反应.
- 对BEM1基因及其在细胞极化中的作用进行遗传分析.
- 对BEM1蛋白 (Bem1p) 的序列分析,以预测功能域.
主要成果:
- 两种新型突变物 (bem1-s) 被鉴定为细胞两极分化的缺陷,特别是在交配期间,而不是植物生长.
- 这些突变者携带BEM1基因的特殊等位基因,该基因对植物细胞两极分化至关重要.
- BEM1蛋白 (Bem1p) 含有SH3域,这表明它在将细胞骨与细胞表面组件联系起作用.
结论:
- 在植物生长和交配期间,BEM1基因在细胞两极分化中发挥着关键的双重作用.
- 特定的BEM1 (bem1-s) 的等位基因对细胞内信号和细胞外信号的偏振反应有不同的影响.
- Bem1p可能作为一个分子桥梁,在极化过程中将细胞骨与细胞表面决定因素连接起来.
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