马管素是细胞周期依赖的微管细胞核形成所需的中心体蛋白质
H C Joshi1, M J Palacios, L McNamara
1Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322.
Nature
|March 5, 1992
概括
马管素对于哺乳动物细胞中微管核形成至关重要. 这种蛋白质是中心体功能和细胞分裂期间适当的螺旋组合所需的.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 细胞骨动力学 细胞骨动力学
背景情况:
- 马管氨酸是管氨酸家族中的一种保存蛋白质.
- 它定位在微管组织中心.
- 玛布林的突变会导致真菌中的线粒性停止.
研究的目的:
- 为了研究在哺乳动物细胞中的玛布林的体内功能.
- 确定马管素在微管核和细胞周期进展中的作用.
主要方法:
- 产生了一种针对玛-氨酸的保守部分的多克隆抗体.
- 在培养的哺乳动物细胞中微注射了抗体.
- 使用免疫光学定位马管,并评估微管的再生和形形成.
主要成果:
- 在整个细胞周期中,抗体成功地与本地中心体结合.
- 在抗体存在时脱聚合后,微管的再生被抑制.
- 在线粒分裂前或期间注射的细胞中,螺旋组件失败了.
结论:
- 在哺乳动物细胞体内微管核形成中,玛布林是不可或缺的.
- 它在中心细胞功能和线粒状形成中起着关键作用.
相关概念视频
Microtubules
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.Microtubules are hollow tubes whose walls are made up of globular tubulin proteins. Each tubulin...
Microtubules
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer. These αβ-heterodimers...
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer. These αβ-heterodimers...
Microtubule Formation
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation of...
Microtubules in Cell Motility
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
Microtubule Instability
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubules in Cell Motility
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...


