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相关概念视频

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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相关实验视频

Updated: Jul 11, 2026

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
04:46

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

充电逆转两动物用于基因传递.

Carla A H Prata1, Yuxing Zhao, Philippe Barthelemy

  • 1Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.

Journal of the American Chemical Society
|September 30, 2004
PubMed
概括

研究人员使用电荷逆转两动物开发了一种新型的基因传递载体. 这种合成载体提高了基因转染效率,与现有的阴离子载体相比.

科学领域:

  • 生物技术是生物技术.
  • 基因治疗 基因治疗
  • 材料科学 材料科学 材料科学

背景情况:

  • 基因疗法在治疗遗传疾病方面具有革命性的潜力.
  • 目前的合成阴离子载体提供优势,但与低基因转染效率作斗争.
  • 需要新的载体设计来克服当前基因传递系统的局限性.

研究的目的:

  • 引入一种新型的电荷逆转两动物,以改善基因传递.
  • 研究新载体的细胞内转化和DNA释放能力.
  • 为了比较电荷逆转两动物的基因转染效率与现有的阴性载体.

主要方法:

  • 一种新的电荷逆转两动物的综合和完整表征.
  • 在两动物和DNA之间形成和分析超分子复合体.
  • 使用开发的载体进行基因转染效率的体外评估.

主要成果:

  • 合成的电荷逆转两动物成功地与DNA形成了超分子复合体.
  • 证实了细胞内从阴离子转化为阴离子状态的变化.
  • 这种新型载体与传统的化两动物相比,显著增强了基因传染.

结论:

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04:46

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

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  • 电荷逆转两动物代表了改善基因传递的有希望的新策略.
  • 两胞体在DNA结合/释放和脂质双层破坏稳定中的双重作用增强了转染.
  • 这种方法为克服当前的基因传递低效率提供了潜在的突破.