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Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening06:00

Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening

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This protocol describes the assembly of a pneumatic system for the delivery of pressurized air to a needle during the process of needle beveling. The protocol further describes the beveling process for creating sharp microinjection needles and how to gauge the relative opening size of the...
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Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels08:12

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This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step.
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This "two-step method" significantly improved the success rate of percutaneous transhepatic choledochoscopy and achieved a better prognosis of intrahepatic and extrahepatic...
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Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)05:39

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Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case...
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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses09:31

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Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal...
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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy11:27

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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research...
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神经区分:TOR和胰岛素受体通路设定了神经区分的节奏.

Pierre Léopold1

  • 1Institute for Signaling, Developmental Biology and Cancer Centre National de la Recherche Scientifique-Parc Valrose, 06108 Nice Cedex 2, France.

Cell
|September 30, 2004
PubMed
概括

拉巴胺素 (TOR) 和胰岛素通路的目标协调动物的生长. 这项研究表明,这些通路也控制了果的神经细胞发育时间,将生长和分化联系起来.

科学领域:

  • 发展生物学 发展生物学
  • 细胞信号传递 细胞信号传递
  • 神经科学是一个神经科学.

背景情况:

  • 拉巴胺素 (TOR) 和胰岛素受体通路的点对于调节多细胞生物 (metazoans) 的生长至关重要.
  • 协调生物体的生长与发育过程,如细胞分化,对于适当的发育至关重要.

研究的目的:

  • 研究拉巴胺素 (TOR) 和胰岛素受体途径在果 (Drosophila) 发育过程中的神经元分化中的标作用.
  • 了解这些保存的信号通路如何将生长控制与发育时间结合起来.

主要方法:

  • 使用Drosophila melanogaster作为一个模型生物.
  • 采用遗传和分子技术来操纵TOR和胰岛素信号.
  • 分析了神经元分化的时间和生长参数.

主要成果:

  • 证明TOR和胰岛素受体通路在调节神经元分化时间方面发挥着新的作用.
  • 表明这些通路对于协调生长速度与神经元发育的进展至关重要.
  • 确定了将新陈代谢状态和生长信号与神经元发育时间联系起来的机制.

结论:

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  • 拉巴胺素 (TOR) 和胰岛素信号通路的目标是Drosophila中神经元分化时间的关键调节者.
  • 这些发现揭示了协调生物生长与发育程序的保存机制,特别是神经元分化.