隔离了编码酵母转录因子IIA子单元的两个基因
1Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Science (New York, N.Y.)
|February 28, 1992
概括
研究人员克隆了酵母TOA1和TOA2基因,这些基因对转录因子IIA (TFIIA) 功能至关重要. 这些基因编码TFIIA子单元,对RNA聚合酶II转录和酵母细胞生长至关重要.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因表达 基因表达
背景情况:
- 转录因子IIA (TFIIA) 是RNA聚合酶II的基底转录机制的关键组成部分.
- 在体外,TFIIA在调节基底转录中发挥作用.
- TFIIA由32个和13.5千多子单位组成.
研究的目的:
- 克隆编码酵母TFIIA亚单元的基因.
- 描述克隆基因及其编码的蛋白质.
- 评估这些基因在酵母中的功能重要性.
主要方法:
- 对TOA1和TOA2的基因克隆.
- 再组合蛋白的表达和净化.
- 在体外转录测试使用酵母和哺乳动物系统.
- 酵母生长试验. 酵母生长试验.
主要成果:
- 编码TFIIA,TOA1和TOA2的32和13.5千多子单元的基因被成功克隆.
- 无论是TOA1还是TOA2,都没有显示出对彼此或已知的基因的序列相似性.
- 重组TFIIA与一个TATA结合蛋白-DNA复合体结合.
- 克隆基因补充了缺乏TFIIA的体外转录系统.
- 发现TOA1和TOA2都对酵母活力至关重要.
结论:
- TOA1和TOA2基因编码了酵母TFIIA的基本子单元.
- 这些基因对于RNA聚合酶II的基底转录至关重要.
- 克隆的基因为进一步研究TFIIA功能和调节提供了工具.
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