盐酸盐对人类热冲击反应的影响
D A Jurivich1, L Sistonen, R A Kroes
1Department of Medicine and Biochemistry, Northwestern University, Evanston, IL 60208.
概括
盐酸激活热冲击转录因子 (HSF) 的DNA结合,但不会触发人体细胞中的热冲击基因表达. 细胞外pH值会影响盐酸盐的HSF激活灵敏度.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 热冲击反应是细胞对压力的防御机制.
- 热冲击转录因子 (HSF) 调节热冲击蛋白 (HSPs) 的表达.
- 酸盐是一种具有已知的细胞效应的抗炎药物.
研究的目的:
- 为了研究盐酸盐对培养人类细胞中热冲击反应的影响.
- 为了确定甲酸盐是否激活热冲击基因转录.
- 探索细胞外pH在酸盐诱导的HSF激活中的作用.
主要方法:
- 培养的人类细胞被用酸盐处理.
- 评估了热冲击转录因子 (HSF) 的DNA结合活性.
- 测量了热冲击基因转录,特别是热冲击蛋白70 (Hsp 70) 的转录.
- 细胞外pH值被调节以检查其对HSF激活的影响.
主要成果:
- 盐酸盐诱导了HSF的DNA结合,达到与热冲击相当的水平.
- 尽管HSF与热冲击元素结合,但甲酸盐没有诱导Hsp 70基因转录.
- 调节细胞外pH值提高了由盐酸盐激活HSF的敏感性.
结论:
- 热冲击基因转录激活是一个复杂的,多步骤的过程.
- HSF DNA 结合是必要的,但不足以通过盐酸盐诱导热冲击基因.
- 细胞外pH值在调节细胞对甲酸盐的反应中起着重要作用.
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