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Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
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大肠杆菌的IspH蛋白:研究铁硫集群的实施和催化

Tobias Gräwert1, Johannes Kaiser, Ferdinand Zepeck

  • 1Contribution from the Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.

Journal of the American Chemical Society
|October 8, 2004
PubMed
概括

过度表达铁硫集群基因显著提高了大肠杆菌IspH酶活性,这对于异类生物合成至关重要. 这种增强需要特定的黄素度,并揭示了IspH蛋白内关键的 [3Fe-4S](+) 集群.

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