在Drosophila早期发育过程中,化表达的状态不是通过克隆传播的
1Department of Biochemistry, University of California, San Francisco 94143-0448.
Cell
|March 6, 1992
概括
在Drosophila胚胎中的细胞谱系追踪显示,对后发育至关重要的化表达,是由靠近无翼信号而维持的,而不是细胞内在的特性. 这表明细胞的命运是由环境决定的.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 血统限制边界定义了Drosophila胚胎中的隔间.
- 嵌入式表达是后部隔间的关键标记.
研究的目的:
- 研究维护嵌入式表达的机制及其在细胞命运决定中的作用.
- 为了确定嵌入式表达是细胞内在的还是受环境调节的.
主要方法:
- 利用一种新的可光激活的谱系追踪器来追踪Drosophila胚胎中的单细胞后代.
- 标记了胚皮阶段的细胞,并分析了克隆组成.
主要成果:
- 没有克隆穿过雕刻的条纹前边 (parasegment边界).
- 嵌入条纹的后部细胞失去了表达,形成混合克隆.
- 条纹前边的稳定刻录表达与接近无翼细胞的关系.
结论:
- 嵌入式表达维护很可能受到细胞外信号无翼的影响,而不仅仅是细胞内在因素.
- 细胞命运的决定可能是一个动态的过程,最初会对细胞环境做出反应.
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