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Updated: Jul 8, 2026

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
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通过生物传感器确定核酸的折叠和展开速度常数. 适用于端粒G-四重复的应用

Yong Zhao1, Zhong-yuan Kan, Zhi-xiong Zeng

  • 1Laboratory of Biochemistry and Biophysics, College of Life Sciences, Wuhan University, PR China.

Journal of the American Chemical Society
|October 14, 2004
PubMed
概括

我们开发了一种新的方法来测量使用表面等离子体共振的核酸折叠和展开速度. 这种技术量化了G-四重复折叠动力学,这对于理解端粒生物学至关重要.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...