骨髓 stromal 细胞分化成心脏表型需要与肌细胞进行细胞间通信
Meifeng Xu1, Maqsood Wani, Yan-Shan Dai
1Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, OH, USA.
Circulation
|October 20, 2004
概括
骨髓 stromal 细胞 (BMSCs) 可以转化为心肌细胞 (肌细胞),当培养与现有的肌细胞. 这种转基因差异化需要直接的细胞间通信,突出显示微环境的重要性.
科学领域:
- 细胞生物学 细胞生物学
- 干细胞研究 干细胞研究
- 心血管科学 心血管科学
背景情况:
- 骨髓 stromal 细胞 (BMSCs) 具有多能分化能力.
- 在特定的环境条件下,BMSCs可能会转化为肌细胞.
- 控制BMSC转化为肌细胞的分子机制尚未完全阐明.
研究的目的:
- 研究细胞微环境在BMSC分化成肌细胞中的作用.
- 为了识别参与BMSC转化为心脏细胞的分子信号.
- 为了确认BMSC转分化为功能性肌细胞.
主要方法:
- 标有绿色光蛋白 (GFP) 的BMSC与新生小鼠心室肌细胞的共同培养.
- 免疫组织化学和电子显微镜用于评估BMSC分化标记物 (例如,α-actinin).
- 逆转录聚合酶链反应 (RT-PCR) 和激光捕获微解剖来分析转基因细胞中的基因表达.
主要成果:
- 与肌细胞共同培养的BMSC表达了α-actinin,并与本地肌细胞形成了间隙结.
- 从肌细胞分开培养的BMSCs没有表现出这些心脏特异性变化.
- 来自BMSCs的转分化肌细胞表达了心脏转录因子,包括GATA-4.
结论:
- 当与肌细胞共同培养时,BMSCs可以成功地转化为表现出心脏表型的细胞.
- 这种转基因分化过程严重依赖于BMSC和肌细胞之间的细胞间通信.
- 像GATA-4这样的心脏转录因子在转化为肌细胞后在BMSC中表达.
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