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Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
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Published on: July 8, 2019

铜 (II) 灭的寡核酸探针用于光DNA传感

Jens Brunner1, Roland Kraemer

  • 1Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.

Journal of the American Chemical Society
|October 21, 2004
PubMed
概括

这项研究介绍了一种新型的分子灯塔,它使用铜 (II) 灭光. 该信标显示,在结合互补DNA时,光度增加了15倍,从而实现了敏感的检测.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...