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Updated: Jul 14, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

破坏稳定的通用链接器用于RNA的自我结合探头的信号放大

Hiroshi Abe1, Eric T Kool

  • 1Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Journal of the American Chemical Society
|October 28, 2004
PubMed
概括

这项研究引入了一种用于寡核酸结合的新分子链接器,使每个目标核酸能够发送多个信号. 这种方法提高了DNA和RNA检测灵敏度,而不需要酶或热循环.

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Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...