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通过NMR扩散测量来绘制HSA药物位点II连接体的皮层映射和竞争性结合

Laura H Lucas1, Kristin E Price, Cynthia K Larive

  • 1Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA.

Journal of the American Chemical Society
|October 28, 2004
PubMed
概括

核磁共振 (NMR) 扩散实验揭示了药物和脂肪酸如何与人血清白蛋白 (HSA) 结合. 这种方法描述了复杂的联结体-蛋白相互作用和三元复合体形成.

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¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
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2D NMR: Overview of Heteronuclear Correlation Techniques

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