凝水凝微球能够精确地提供基本纤维细胞生长因子,用于功能附带血管的发展
Akihiro Hosaka1, Hiroyuki Koyama, Toshihiro Kushibiki
1Department of Vascular Regeneration, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Circulation
|November 3, 2004
概括
酸性凝水凝微球 (AGHM) 通过动脉内注射有效地提供基本纤维细胞生长因子 (bFGF),以促进缺血肢体的附带血管发育. 这种新型的输送系统显示了血管封闭性疾病的治疗潜力.
科学领域:
- 生物材料科学是生物材料的科学.
- 血管生物学 血管生物学
- 再生医学是一种再生医学.
背景情况:
- 在血管封闭性疾病中,生长因子对附带血管发育至关重要.
- 对增长因素的有效交付系统仍然是一个挑战.
- 酸性凝水凝微球 (AGHM) 已开发,以结合基本纤维细胞生长因子 (bFGF).
研究的目的:
- 为了研究bFGF浸AGHMs的动脉内 (IA) 管理的疗效.
- 为了确定bFGF是否可以从被困在远端血管中的AGHMs中释放出来,以诱导动脉生成.
- 促进功能附带血管的发育,确保血液供应.
主要方法:
- 各种大小的AGHM 含有125I标记的bFGF被注射到后肢缺血的子的左侧内动脉中.
- 在缺血后肢的放射性积累被测量为不同的AGHM大小.
- 在注射后评估了小牛的血压和区域血流.
- 在29微米直径的bFGF浸AGHMs的IA给药28天后,评估了附带血管功能.
主要成果:
- 29和59微米直径的AGHM在缺血后肢中显示了大约80%的放射性积累,与10微米AGHM不同.
- 对29微米直径的bFGF浸AGHM的IA管理显著改善了担保船舶的发展.
- 在IA给药后立即没有观察到血压或区域血流的显著变化.
结论:
- 对29微米直径的bFGF浸的AGHM进行IA的管理有效地诱导了功能性附带血管.
- 这种方法可以促进动脉形成,而不会加剧缺血症.
- 与bFGF浸的AGHM方法对血管封闭性疾病具有治疗前景.
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