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Manufacture and Drug Delivery Applications of Silk Nanoparticles
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使用离子液体溶解和再生Bombyx mori丝纤维蛋白
David M Phillips1, Lawrence F Drummy, Deborah G Conrady
1Materials & Manufacturing Directorate, Air Force Research Laboratory, 2941 Hobson Way, Wright-Patterson AFB, Ohio 45433, USA.
Journal of the American Chemical Society
|November 4, 2004
概括
离子液溶剂有效地溶解和再生丝虫的丝. 离子选择和冲洗溶剂显著影响再生的丝薄膜.
科学领域:
- 材料科学 材料科学 材料科学
- 生物材料工程 生物材料工程
- 聚合物化学 聚合物化学
背景情况:
- 丝虫丝 (Bombyx mori) 是一种具有独特特性的天然蛋白质纤维.
- 开发有效的丝溶解和再生方法对于先进的应用至关重要.
- 离子液体为生物聚合物提供了作为环境良好的溶剂的潜力.
研究的目的:
- 评估基于伊米达的离子液体,用于溶解和再生丝丝.
- 为了研究离子液体的离子对丝溶性的影响.
- 研究再生丝膜的结构和地形变化.
主要方法:
- 在各种基于imidazolium的离子液体中溶解Bombyx mori丝.
- 使用广角X射线散射 (WAXS) 确认丝溶解.
- 将溶解的丝再生成100微米厚的薄膜.
- 在用乙或甲醇冲洗后分析膜拓和蛋白质二次结构.
主要成果:
- 离子液的离子显著影响丝的溶解性.
- 广角X射线散射证实了丝的成功溶解.
- 再生的丝薄膜呈现出不同的地形和次要结构,取决于冲洗溶剂 (乙尼和甲醇).
- 在从1-基-3-甲基利米达化物再生的薄膜中观察到双重破裂.
结论:
- 基于伊米达的离子液体是丝虫丝溶解和再生的合适溶剂.
- 离子液体离子的选择对于实现丝溶解度至关重要.
- 再生后的加工,特别是冲洗溶剂,对最终的薄膜性质产生深远的影响,包括蛋白质结构和表面地形.
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