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相关概念视频

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
UV–Vis Spectrum01:30

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

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相关实验视频

Updated: Jul 7, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

在合成四螺旋束蛋白中产生的不同类型的铜中心的光谱识别.

Robert Schnepf1, Wolfgang Haehnel, Karl Wieghardt

  • 1Max-Planck-Institut für Bioanorganische Chemie, Stiftstrasse 34-36, D-45470 Mülheim a. d. Ruhr, Germany. rs@complex-biosystems.com

Journal of the American Chemical Society
|November 4, 2004
PubMed
概括

研究人员使用理性组合方法创建了稳定的合成铜结合蛋白. 这些工程蛋白质表现出增强的稳定性和特异性,使得各种铜结合点的详细表征成为可能.

科学领域:

  • 生物化学 生物化学
  • 蛋白质工程是指蛋白质工程.
  • 生物有机化学 生物有机化学

背景情况:

  • 模板组装合成蛋白质为设计金属蛋白提供了一个平台.
  • 控制蛋白质支架内的金属离子协调对于功能至关重要.
  • 以前的设计显示出希望,但需要提高稳定性和特异性.

研究的目的:

  • 设计具有特定铜结合点的稳定合成四螺旋束蛋白质.
  • 调查序列变化如何影响金属蛋白稳定性和铜中心特性.
  • 描述工程铜结合点的结构和电子特性.

主要方法:

  • 组合的理性和组合蛋白质设计方法.
  • 180种不同的四螺旋捆的合成和表征.
  • 包括UV-Vis,EPR和CD在内的光谱分析用于研究铜协调.

主要成果:

  • 大约90%的合成蛋白质成功与高特异性结合铜.
  • 金属蛋白稳定性增加了多达两个数量级.
  • 鉴定了三种不同的铜结合部位类型 (I型,II型和Cu (A) 模仿) 由硬质因子控制.

结论:

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Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
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Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

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Last Updated: Jul 7, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
08:26

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)

Published on: August 31, 2018

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

  • 结构多样化和稳定的铜结合点可以成功地被设计成四螺旋束蛋白质.
  • 在His2Cys连接体组附近的固态影响对于决定协调几何学至关重要.
  • 这项工作为设计具有定制功能的新型金属蛋白提供了基础.