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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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相关实验视频

Updated: May 7, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

通过微处理器复合体处理初级microRNAs.

Ahmet M Denli1, Bastiaan B J Tops, Ronald H A Plasterk

  • 1Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.

Nature
|November 9, 2004
PubMed
概括
此摘要是机器生成的。

含有Drosha和Pasha的微处理器复合体对于微RNA (miRNA) 成熟至关重要. 帕沙 (Pasha) 是一个非常有价值的人.

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相关实验视频

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

mirMachine: A One-Stop Shop for Plant miRNA Annotation
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mirMachine: A One-Stop Shop for Plant miRNA Annotation

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 微RNAs (miRNAs) 是基因表达的关键转录后调节者.
  • 成熟的miRNAs从初级miRNA转录 (pri-miRNAs) 通过两步路径进行处理.
  • 德罗莎是一种RNase III核酶,在核中启动了primiRNA处理.

研究的目的:

  • 为了研究含有Drosha的微处理器复合物的蛋白质成分.
  • 阐明Pasha (Drosha的合作伙伴) 在miRNA生物发生和功能中的作用.

主要方法:

  • 微处理器复合体的生物化学特征.
  • 功能性研究涉及抑制Drosophila和Caenorhabditis elegans中的帕沙表达.
  • 对primiRNA和成熟miRNA水平的分析.
  • 在C. elegans中进行记者测定和表型分析.

主要成果:

  • 德罗沙的功能是多蛋白质复合体,即微处理器的一部分.
  • 帕沙是一种双链RNA结合蛋白,是微处理器复合物的组成部分.
  • 帕沙衰竭或突变会损害primiRNA处理,降低成熟miRNA水平,并影响基因调节.

结论:

  • 帕沙在miRNA成熟过程中起着至关重要的作用.
  • 包括帕沙在内的微处理器复合体对于miRNA介导的基因调节至关重要.
  • 帕沙在miRNA生物生成中的功能在物种之间得到保护.