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RNA Secondary Structure Prediction Using High-throughput SHAPE
Published on: May 31, 2013
She2p是一种新型RNA结合蛋白,具有基本的螺旋状发针图案
Dierk Niessing1, Stefan Hüttelmaier, Daniel Zenklusen
1Laboratories of Molecular Biophysics and The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Cell
|November 13, 2004
概括
研究人员阐明了She2p的结构,She2p是一种对酵母中不对称的mRNA运输至关重要的蛋白质. 这一发现揭示了一种新型的核酸结合蛋白,并阐明了ASH1 mRNA运输机制.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 结构生物学 结构生物学
背景情况:
- 在核糖核蛋白颗粒 (mRNP) 中选择性mRNA传输对于真核细胞中不对称的信息分布至关重要.
- 酵母中ASH1mRNA的actin-dependent运输是mRNP转位的一个研究完善的模型.
- 形成ASH1 mRNP复合体涉及RNA结合蛋白She2p识别特定的邮政编码元素.
研究的目的:
- 确定RNA结合蛋白 She2p.p. 的X射线结构.
- 描述She2p的RNA结合特性和机制.
- 阐明 She2p 在启动 ASH1 mRNP 复杂组合中的作用.
主要方法:
- 在1.95A分辨率下测定She2p的结构的X射线晶体学.
- 在体外生化测试以评估RNA结合亲和力和二分体依赖性.
- 在实验室和体内绘制She2p.p上的RNA结合表面的映射.
主要成果:
- X射线结构揭示了She2p作为一种新型的核酸结合蛋白,形成一个对称的同位体.
- She2p表现出强大的,二分体依赖的RNA结合.
- 该RNA结合表面被映射到一个基本的螺旋式针头,为mRNA识别提供了洞察力.
结论:
- She2p代表了一种具有独特二维结构的新一类核酸结合蛋白.
- 分化对于She2p强大的RNA结合活性至关重要.
- 已经提出了一种用于mRNA依赖的ASH1 mRNP组合的启动机制,其中包括She2p.
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