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Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
甲氧化酶催化化海洋天然产品的生物合成
Jayme N Carter-Franklin1, Alison Butler
1Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106-9510, USA.
Journal of the American Chemical Society
|November 19, 2004
概括
海洋红藻产生独特的化化合物. 来自红藻的氧化酶 (V-BrPO) 已被证明是第一个催化不对称化和烯循环的酶,形成生物活性天然产品.
科学领域:
- 海洋天然产品生物合成
- 酵素化酶的使用
- 类物质的化学成分
背景情况:
- 海洋红藻是生物活性化天然产品的丰富来源.
- 烯作为许多复杂代谢物的生物原始前体.
- 了解循环化烯的生物合成具有重要意义.
研究的目的:
- 为了研究海洋红藻中烯化和循环的酶机制.
- 为了确定负责循环化基二烯生物合成的特定酶.
- 确定瓦氧化酶 (V-BrPO) 在生产这些海洋天然产品中的作用.
主要方法:
- 从海洋红藻 (Laurencia,Plocamium,Corallina) 中分离和描述V-BrPO.
- 使用塞斯基二烯 (E) - - - +) - 尼罗利多尔作为基质的酶分析.
- 使用光谱方法分析反应产物,包括α,β,gamma-snyderol和 (+) - 3β-bromo-8-epicaparrapi氧化物.
- 酵素产品与通过使用2,4,4,6-tetrabromocyclohexa-2,5-dienone (TBCO) 的化学合成获得的产品的比较.
主要成果:
- V-BrPO催化了 (E) - ((+)) - 内罗利多尔的不对称化和循环化.
- 这种酶产生特定的snyderols和-epicaparrapi氧化物的二聚体.
- 这些产品,包括马-斯尼德罗尔作为拟议的中间体,在各种海洋红藻物种中发现.
- 与化学合成相比,酶反应产生更高的二聚体选择性.
结论:
- 已确定V-BrPO是第一个催化海洋红藻中化循环基二烯的生物合成的酶.
- 该酶可能通过产生与基底反应的离子中间体来起作用.
- 这项研究阐明了形成有价值的海洋自然产品的关键步骤.
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