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Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
Published on: June 29, 2011
通过c-myc蛋白诱导纤维细胞中的亡
G I Evan1, A H Wyllie, C S Gilbert
1Imperial Cancer Research Fund Laboratories, London, England.
Cell
|April 3, 1992
概括
纤维细胞中c-myc蛋白的构成性表达会诱导细胞亡,或编程细胞死亡,而不是不受控制的生长. 较高的c-myc水平增加了对这种细胞死亡的敏感性.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 在老鼠-1纤维细胞中,c-myc瘤基因的构成表达在低血清条件下防止生长停止.
- 尽管有持续的增殖信号,但由于显著的细胞死亡,这些细胞不会积累.
研究的目的:
- 为了研究在有放松c-myc表达的纤维细胞中观察到的细胞死亡机制.
- 确定c-myc的亡功能与其其他已知的细胞作用之间的关系.
主要方法:
- 在血清剥夺下对具有构成性c-myc表达的老鼠-1纤维细胞细胞死亡的分析.
- 参与诱导亡的c-myc蛋白区域的表征.
- 在细胞周期的各个阶段和制方法中,对具有放松c-myc的细胞中细胞亡的评估.
主要成果:
- 在c-myc表达纤维细胞中,细胞死亡依赖于c-myc蛋白,并通过亡发生.
- 诱导亡所需的c-myc蛋白的区域与共转化,自我调节和分化抑制所需的区域重叠.
- 较高水平的c-myc蛋白与在血清剥夺后对亡的敏感性增加相关.
- 放松调节的c-myc表达触发了不同制方法和细胞周期阶段的增长停止细胞的亡.
结论:
- c-myc蛋白在诱导细胞亡中起着至关重要的作用,作为细胞命运的关键调节者.
- c-myc的亡功能与其其他致癌和调控功能密切相关.
- 放松调节的c-myc表达可以覆盖细胞循环检查点,并诱导受程序的细胞死亡,这表明它在癌症发展中起着复杂的作用.
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