近红外光显微镜的单壁碳纳米管在细胞细胞
Paul Cherukuri1, Sergei M Bachilo, Silvio H Litovsky
1Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA.
Journal of the American Chemical Society
|December 2, 2004
概括
单壁碳纳米管很容易被巨细胞吸收,并积聚在细胞内囊泡中. 这种吸收是通过细胞酶发生的,不会影响巨细胞群体的增长.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 细胞生物学 细胞生物学
背景情况:
- 单壁碳纳米管 (SWCNTs) 是具有独特特性的纳米材料.
- 了解它们与生物系统的相互作用对于安全和应用至关重要.
研究的目的:
- 为了研究原始SWCNTs进入巨类细胞的吸收机制.
- 评估SWCNTs对巨细胞活力和繁殖的影响.
主要方法:
- 利用SWCNTs的内在近红外光用于跟踪.
- 使用近红外光显微镜 (波长大于1100nm).
- 在96小时内监测了巨细胞种群的增长.
主要成果:
- 在巨细胞中,SWCNTs表现出特有的光光谱.
- 光强度与化时间和SWCNT度相关.
- 高对比度成像揭示了SWCNT在细胞内囊泡中的定位.
- 发细胞被确定为主要的吸收机制.
- 在高达4μg/ml的SWCNT度下,巨细胞生长在96小时内保持不变.
结论:
- 纯净的SWCNTs通过细胞分裂被巨细胞有效地内化.
- 在细胞内囊泡中积聚SWCNTs.
- 长细胞的活力和增殖不会因短期暴露于相关SWCNT度而受到损害.
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