相关实验视频
Updated: Jul 10, 2026
22:27
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
基因组的物理地图
John W Wallis1, Jan Aerts, Martien A M Groenen
1Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA.
Nature
|December 14, 2004
概括
使用指纹克隆映射创建了基因组的新物理地图. 这种20倍覆盖范围的地图有助于验证基因组组装和功能基因组学研究.
科学领域:
- 基因组学就是基因组学.
- 进行比较基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 基因组组装策略结合了全基因组猎枪测序和层次地图辅助测序.
- 整个基因组图,从细胞遗传到序列分辨率,对于精确的基因组组件至关重要.
- 指纹克隆地图提供有序的重叠克隆,用于验证和定基因组序列.
研究的目的:
- 以克隆为基础的基因组 (Gallus gallus) 的物理地图.
- 为验证基因组序列草案提供资源,并促进功能基因组研究.
- 通过将克隆与其他测序基因组对齐,使比较基因组学成为可能.
主要方法:
- 使用指纹技术构建基于克隆的物理地图.
- 使用限制酶来消化代表基因组的克隆.
- 通过重叠的克隆实现了基因组的20倍覆盖.
主要成果:
- 创建了一个物理地图,其中包括260个重叠克隆的连接.
- 这张地图覆盖了大约91%的基因组.
- 能够识别与其他测序基因组中的位置对齐的克隆.
结论:
- 呈现的物理地图是Gallus gallus基因组的宝贵资源.
- 这张地图有助于验证基因组组装顺序和缩小序列缺口.
- 该地图促进了未来的功能基因组研究和比较分析.
相关概念视频
02:07Organization of Genes
Overview
02:07Organization of Genes
Overview
02:11Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
01:09Epistasis Analysis
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
02:11Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
01:20Genetic Material
Within the human body, a complex and detailed system of trillions of cells works in unison to sustain life. Each cell houses a nucleus, which contains 46 chromosomes divided into 23 pairs. Chromosomes are highly coiled structures made of the genetic material DNA. These chromosomes are essential carriers of genetic information, with half inherited from the mother through her egg and the other half from the father's sperm, combining to create the unique genetic makeup of an individual.