相关实验视频
Updated: Jul 16, 2026

09:21
Microcontact Printing of Proteins for Cell Biology
Published on: December 5, 2008
在按需打印机中的蛋白质损伤
Gary M Nishioka1, Andrea A Markey, Charles K Holloway
1H & N Instruments, Inc., PO Box 4338, Newark, Ohio 43055, USA. HNI@Infinet.com
Journal of the American Chemical Society
|December 17, 2004
概括
由于快速压缩,喷墨印刷可能会损害像过氧化酶这样的酶. 将三糖和葡萄糖等糖类添加到溶液中,有助于在这个过程中保护酶.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 喷墨印刷是一种合成微阵列的多功能方法.
- 了解印刷对生物分子的影响对于微阵列应用至关重要.
研究的目的:
- 为了研究在喷墨打印过程中对模型酶过氧化酶造成的损伤.
- 评估特定添加剂对酶完整性的保护作用.
主要方法:
- 使用喷墨打印技术来沉积含有过氧化酶的溶液.
- 通过分析方法特征化酶损伤 (摘要中未指定细节).
- 评估了三糖和葡萄糖对印刷后酶稳定性的影响.
主要成果:
- 喷墨打印过程会对过氧化酶造成重大损害.
- 添加三和葡萄糖显然可以减轻这种印刷引起的损伤.
- 糖在印刷的压缩阶段作为酶的保护剂.
结论:
- 喷墨印刷对微阵列中的酶稳定性构成风险.
- 三糖和葡萄糖是有效的辅助剂,可以在印刷的微阵列中保存酶功能.
- 这一发现对开发更强大的喷墨打印的基于酶的生物传感器和诊断工具有影响.
相关概念视频
Overview of DNA Repair
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Chemically...
Translesion DNA Polymerases
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...

