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相关实验视频

Updated: Jul 13, 2026

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
12:19

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Published on: May 27, 2012

无标签的分子相互作用测定使用纳米级干涉测量.

Dmitry A Markov1, Kelly Swinney, Darryl J Bornhop

  • 1Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235-1822, USA.

Journal of the American Chemical Society
|December 17, 2004
PubMed
概括

这项研究引入了矩形通道 (BIRC) 中的反向散射干扰计,用于在皮科升体积中无标签量化分子相互作用. BIRC 能够对结合事件进行敏感的,非侵入性的检测,进步细胞功能和治疗研究.

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科学领域:

  • 生物物理学的生物物理.
  • 分析化学 分析化学
  • 微流体学 微流体学

背景情况:

  • 对细胞生物学和药物开发来说,量化蛋白质-蛋白质和配体-基质相互作用至关重要.
  • 无标签测试是模拟生物条件的首选,但在微流体集成和低体积灵敏度方面面临挑战.
  • 现有的方法在不损害灵敏度的情况下努力减少检测量,以实现通用分析物量化.

研究的目的:

  • 开发一种方法,在微流体器件中以皮科升体积量量化分子相互作用的无标签量化.
  • 为了证明在矩形通道 (BIRC) 中反散干涉测量的能力,以进行敏感和非侵入式检测.
  • 为克服微流体应用现有的无标签测量工具的局限性.

主要方法:

  • 使用反向散射干涉测量用于在聚二甲基西洛 (PDMS) 中成型的矩形微流体通道 (BIRC).
  • 在225 pL检测体积内使用低功率连贯辐射进行光学检测.
  • 量化了不可逆转的斯特雷普塔维丁-生物素结合和可逆的蛋白质A-人类IgG Fc相互作用.

主要成果:

  • 生物的检测极限为47 x 10^-15 mol,IgG Fc.的检测极限为2 x 10^-15 mol.
  • 在微流体通道中使用干扰测量证明了高灵敏度的通用溶解物量化.
  • 成功完成了可逆分子相互作用的无标签量化,具有女性分子敏感性.

结论:

  • BIRC为高灵敏度的picoliter体积的无标签分子相互作用研究提供便利.
  • 该方法与廉价的PDMS微流体芯片和低功耗光学相容.
  • BIRC显示了在微流体系统中高通量结合亲和度量化的潜力.

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Last Updated: Jul 13, 2026

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
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Published on: May 27, 2012

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Published on: July 18, 2014

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Measuring the Interaction Force Between a Droplet and a Super-hydrophobic Substrate by the Optical Lever Method

Published on: June 14, 2019