染色质结构的NAD+依赖调节和通过PARP-1的核细胞结合特性转录的转录
Mi Young Kim1, Steven Mauro, Nicolas Gévry
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Cell
|December 21, 2004
概括
聚 ((ADP-ribose) 聚合酶1 (PARP-1) 结合了核细胞,紧缩了染色体,抑制了转录. 这种酶活性由核细胞刺激,并由PARG逆转,影响基因调节.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 生物化学 生物化学
背景情况:
- 聚ADP-ribose) 聚合酶1 (PARP-1) 是一个关键酶,参与DNA修复和基因调节.
- PARP-1催化了ADP-ribose单元从NAD+转移到目标蛋白的过程.
研究的目的:
- 为了研究PARP-1的以前未被描述的核细胞体结合特性.
- 阐明PARP-1在调节染色质结构和转录抑制中的作用.
主要方法:
- 描述PARP-1与核细胞相互作用的特征.
- 对PARP-1的NAD+依赖自动修饰活动的分析.
- 在体内研究以评估PARP-1在染色质域中的局部化.
主要成果:
- PARP-1 特别与核细胞结合,促进紧和转录抑制的染色体结构.
- 核细胞组强烈刺激PARP-1的自动修饰活动,导致其从染色质中释放出来.
- PARP-1的活性是NAD+依赖的,由多 (ADP-ribose) 糖酶 (PARG) 逆转,并被ATP抑制.
结论:
- PARP-1的功能既是结构部件,也是染色质结构的调节器.
- 在体内,PARP-1的酶活性有助于转录抑制的染色质域的形成.
- 这些PARP-1-关联的抑制域与由基因素H1或活性转录调节的区别很大.
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