系统地识别和分析异声拼接沉声器
Zefeng Wang1, Michael E Rolish, Gene Yeo
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Cell
|December 21, 2004
概括
研究人员使用报道系统识别出了141个外来拼接声器 (ESS). 这些ESS通过抑制拼接部位来调节基因表达,影响替代拼接和基因功能.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物信息学是一种生物信息学.
背景情况:
- 异声拼接沉声器 (ESS) 是抑制拼接部位识别的监管元件.
- ESS在替代拼接 (AS) 中发挥作用,这一过程增加了蛋白质组的多样性.
- 了解ESS功能对于破译基因调节至关重要.
研究的目的:
- 系统地识别出新的外声拼接沉声器 (ESS).
- 描述已识别的ESS的动机和潜在功能.
- 研究ESS在构成性和替代性拼接中的作用.
主要方法:
- 开发一种用于高通量查的体内剪接报告系统.
- 随机分核酸库的选,以确定ESS活动.
- 用ExonScan对ESS活动在异质环境中的验证和生物信息分析.
主要成果:
- 识别了141个外显拼接声器 (ESS) 摄像机,具有133个独特的序列.
- 在不同细胞环境中,确认了十多个已识别的ESS的沉声器活动.
- 聚合独特的ESS十度室成七个假定图案,其中一些类似于已知的hNRNP结合点.
结论:
- 该研究确定了许多新的ESS,扩大了已知的拼接监管元素的作品集.
- 已识别的ESS动机涉及伪子抑制,拼接部位定义和替代拼接.
- 这些发现提供了通过拼接控制管理基因表达的调节机制的见解.
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