基本酵母核糖体蛋白激活基因ifh1的增长调节的招募
Stephan B Schawalder1, Mehdi Kabani, Isabelle Howald
1Department of Molecular Biology and NCCR Program Frontiers in Genetics University of Geneva, Sciences III, 30, quai Ernest-Ansermet, Geneva 4, CH-1211, Switzerland.
Nature
|December 24, 2004
概括
基本蛋白质Ifh1在最佳生长条件下激活酵母中的核糖体蛋白 (RP) 基因促进体. 如果h1结合由Fhl1调节,在压力时减少,影响细胞生长控制.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 核糖体生物生成对于细胞生长控制至关重要.
- 核糖体蛋白 (RP) 基因在酵母中被高度转录,但在压力期间会降低调节.
- 像TOR这样的信号通路调节RP基因转录,但启动控制不清楚.
研究的目的:
- 为了研究Saccharomyces cerevisiae中RP基因启动的调节.
- 在各种条件下确定参与控制RP基因转录的关键蛋白质.
主要方法:
- 染色体免疫沉测试以评估蛋白质与RP基因促进体的结合.
- 分析Ifh1和Fhl1的互动和招聘情况.
- 在TOR抑制,营养物质枯竭和饥饿释放条件下研究RP基因调节.
主要成果:
- 基本蛋白质Ifh1与酵母中的许多RP基因促进体结合并激活它们.
- 如果h1通过Fhl1的分叉关联域被招募到RP基因促进器中.
- 在TOR抑制和营养物质耗尽期间,ifh1结合减少,并在饥饿释放时恢复.
结论:
- 在最佳生长条件下,ifh1在激活RP基因促进剂方面发挥着核心作用.
- Fhl1调解了Ifh1对RP基因促进者的招募.
- Ifh1和Fhl1是RP基因表达的关键调节者,将环境线索与核糖体生物发生联系起来.
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