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相关概念视频

The Central Dogma01:25

The Central Dogma

Overview
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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相关实验视频

Updated: Jul 6, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

基于纳米晶体的单核酸多态的生物电子编码.

Guodong Liu1, Thomas M H Lee, Joseph Wang

  • 1Department of Chemical Engineering, Biodesign Institute, Arizona State University, Tempe, Arizona 85287-6006, USA.

Journal of the American Chemical Society
|January 6, 2005
PubMed
概括

这项研究引入了一种使用纳米晶体检测单核酸多态 (SNP) 的新生物电子方法. 这种技术能够快速,低成本,高通量选基因突变.

科学领域:

  • 生物电子学 生物电子学
  • 纳米技术纳米技术
  • 遗传学 遗传学是一种遗传学.

背景情况:

  • 单核酸多态性 (SNP) 是影响疾病易感性的常见遗传变异.
  • 精确有效地检测SNP对于遗传研究和诊断至关重要.
  • 现有的SNP检测方法可能是复杂的,昂贵的,或缺乏高吞吐量.

研究的目的:

  • 开发一种新的生物电子方法,用于编码和检测未知的单核酸多态 (SNP).
  • 利用编码纳米晶体通过基配对和电压分析进行SNP识别.
  • 为SNP建立一个快速,简单,低成本和高通量选协议.

主要方法:

  • 一种生物电子方法,采用四种不同的纳米晶体 (ZnS,CdS,PbS,CuS) 与特定的核酸结合 (腺,细胞,瓜诺,胺).
  • 连续引入纳米晶-单核酸合物到DNA混合涂层磁珠中.
  • 分析由基配不匹配产生的特征性的多电位伏特ammograms,通过剥离电压计放大.
  • 在一次电压测量运行中检测最多8个可能的单基不匹配.

主要成果:

  • 成功识别单个单基不匹配基于独特的伏特图的峰值潜力.
  • 使用纳米晶体追踪器在单个DNA点中检测出两个已知的突变的演示.

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Last Updated: Jul 6, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

  • 该方法为每个类型的SNP提供了独特的电化学特征.

    • 结论:

    • 开发的生物电子方法为SNP检测提供了一种敏感和特定的方法.
    • 使用编码纳米晶体和电压测量有助于快速和经济高效的SNP选.
    • 该协议有可能显著推进高通量遗传分析和诊断.