异常的连接物歧视由一个与疏水域连接的血红蛋白复合的肌红蛋白
Hideaki Sato1, Masahiro Watanabe, Yoshio Hisaeda
1Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan.
Journal of the American Chemical Society
|January 6, 2005
概括
化学修饰的肌球蛋白显示出比一氧化碳更强的氧气选择性. 引入一种疏水域改善了O2结合和稳定,增加了连接体选择性而不改变氨基酸序列.
科学领域:
- 生物化学 生物化学
- 蛋白质工程是指蛋白质工程.
- 频谱学是一种光谱学.
背景情况:
- 肌球蛋白的血口袋对于氧气和一氧化碳的结合至关重要.
- 了解肌球蛋白中的配体选择性是蛋白质功能研究的关键.
研究的目的:
- 为了构建和表征复制的马心肌球蛋白与修改的血 propionate 侧链.
- 研究一种新型疏水性域对连接体结合动力学和选择性的影响.
主要方法:
- 激光闪光灯的光解法.
- 停止流速快速混合技术的技术.
- 血 propionate 侧链的化学修饰
主要成果:
- 具有疏水性域的复合肌球蛋白显示增加了O2稳定性.
- 二氧化碳分离率增加了20倍,表明远部位扰动.
- 实现了O2对CO的选择性显著增加 (M'值=0.88).
结论:
- 血 propionates 的化学修饰可以增强肌球蛋白的 O2 选择性.
- 疏水性域的结合扰乱了远部位结构,使O2比CO更受欢迎.
- 这种方法可以提高O2的选择性,而不会改变氨基酸残留.
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