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Updated: Jul 11, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

使用绿色光蛋白片段重组陷检测蛋白质-蛋白质相互作用:范围和机制

Thomas J Magliery1, Christopher G M Wilson, Weilan Pan

  • 1Department of Molecular Biophysics & Biochemistry, Yale University, P.O. Box 208114, New Haven, Connecticut 06520-8114, USA.

Journal of the American Chemical Society
|January 6, 2005
PubMed
概括

这项研究提出了一种新的方法,用于检测使用绿色光蛋白片段的蛋白质-蛋白质相互作用. 屏幕有效地识别弱和短暂的相互作用,帮助蛋白质组分析.

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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer11:46

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

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Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.