Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Strong Acid and Base Solutions03:22

Strong Acid and Base Solutions

A strong acid is a compound that dissociates completely in an aqueous solution and produces a concentration of hydronium ions equal to the initial concentration of acid. For example, 0.20 M hydrobromic acid will dissociate completely in water and produces 0.20 M of hydronium ions and 0.20 M of bromide ions.
Determining the pH of Salt Solutions04:08

Determining the pH of Salt Solutions

The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution. In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
Acid Strength and Molecular Structure03:05

Acid Strength and Molecular Structure

Binary Acids and Bases
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
Calculating pH Changes in a Buffer Solution02:45

Calculating pH Changes in a Buffer Solution

A buffer can prevent a sudden drop or increase in the pH of a solution after the addition of a strong acid or base up to its buffering capacity; however, such addition of a strong acid or base does result in the slight pH change of the solution. The small pH change can be calculated by determining the resulting change in the concentration of buffer components, i.e., a weak acid and its conjugate base or vice versa. The concentrations obtained using these stoichiometric calculations can be used...
Standard Electrode Potentials03:02

Standard Electrode Potentials

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
Ladder Diagrams: Redox Equilibria01:30

Ladder Diagrams: Redox Equilibria

Ladder diagrams are useful tools for understanding redox equilibrium reactions, especially the effects of concentration changes on the electrochemical potential of the reaction. The vertical axis in the redox ladder diagrams represents the electrochemical potential, E. The area of predominance is demarcated using the Nernst equation.
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Towards a dual copper(II) and iron(III) transmetalation strategy for an anticancer application with the deferasirox chelator.

Journal of inorganic biochemistry·2026
Same author

Ligand-Based Redox Chemistry and Anti-Kasha Fluorescence in Silver(I) Tripyrrindione Radical.

Inorganic chemistry·2026
Same author

Periodic fasting and refeeding re-shapes lipid saturation, storage, and distribution in brown adipose tissue.

PLoS biology·2026
Same author

Trivalent chromium interacts directly with acetylated lysine.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2025
Same author

Compressive Force Activation of the Neuronal Nitric Oxide Synthase Enzyme.

ACS omega·2025
Same author

Detection of a clamp-shaped conformation of a neuronal nitric oxide synthase construct by pulsed EPR.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2025

相关实验视频

Updated: Jul 5, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

17O ESEEM证据证明了硫酸盐氧化酶的高pH形式的中心的轴性氧化交换.

Andrei V Astashkin1, Changjian Feng, Arnold M Raitsimring

  • 1Department of Chemistry, University of Arizona, Tucson, Arizona 85721-0041, USA.

Journal of the American Chemical Society
|January 13, 2005
PubMed
概括
此摘要是机器生成的。

通过先进的光谱学,在硫酸盐氧化酶中发现了一种新的氧联体. 这一发现为酶的活性部位,特别是中心提供了新的见解.

更多相关视频

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

A Dual-Functional Electroactive Filter Towards Simultaneously Sb(III) Oxidation and Sequestration
08:34

A Dual-Functional Electroactive Filter Towards Simultaneously Sb(III) Oxidation and Sequestration

Published on: December 5, 2019

相关实验视频

Last Updated: Jul 5, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

A Dual-Functional Electroactive Filter Towards Simultaneously Sb(III) Oxidation and Sequestration
08:34

A Dual-Functional Electroactive Filter Towards Simultaneously Sb(III) Oxidation and Sequestration

Published on: December 5, 2019

科学领域:

  • 生物化学 生物化学
  • 生物有机化学 生物有机化学
  • 频谱学是一种光谱学.

背景情况:

  • 硫酸盐氧化酶是硫代谢中的关键酶.
  • 了解硫酸盐氧化酶的活性位点对于阐明其催化机制至关重要.
  • 之前的电子磁共振 (EPR) 研究已经为中心提供了洞察力.

研究的目的:

  • 用先进的光谱技术研究硫酸盐氧化酶的高pH形式.
  • 识别和表征与中心相关的可交换氧联结体.
  • 为了更深入地了解Mo(V) 中心的电子结构和协调环境.

主要方法:

  • 使用了170电子电子双共振 (ESEEM) 谱学.
  • 在29.25 GHz使用超精细亚级相关性 (HYSCORE) 光谱.
  • 分析了中心与氧联体的超细相互作用.

主要成果:

  • 鉴定了一种新型的可交换的17O联结体,这种联结体是硫酸盐氧化酶的高pH形式.
  • 与此前检测到的体相比,这种新的氧体表现出明显较小的超细相互作用 (大约5 MHz).
  • 新发现的配体被分配到Mo(V) 中心的轴性氧基组.

结论:

  • 17O ESEEM HYSCORE研究成功地表征了一种以前未被检测到的可交换氧联结体.
  • 这一发现完善了我们对硫酸盐氧化酶中中心的协调环境和电子性质的理解.
  • 这些结果有助于建立一个更全面的酶催化机制模型,特别是在高pH条件下.