Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

β-Sitosterol β-D-glucoside (BSSG) triggers intestinal inflammation in zebrafish and mouse models prior to neurodegeneration onset.

Journal of biomedical science·2026
Same author

Nuclear ASC speck formation in microglia is associated with inflammasome priming and is exacerbated in LRRK2-G2019S Parkinson's disease.

Neurobiology of disease·2025
Same author

Beyond neurons: How does dopamine signaling impact astrocytic functions and pathophysiology?

Progress in neurobiology·2025
Same author

Correction: Nanorod-associated plasmonic circular dichroism monitors the handedness and composition of α-synuclein fibrils from Parkinson's disease models and post-mortem brain.

Nanoscale·2024
Same author

PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner.

Cell death & disease·2024
Same author

Nanorod-associated plasmonic circular dichroism monitors the handedness and composition of α-synuclein fibrils from Parkinson's disease models and post-mortem brain.

Nanoscale·2024

相关实验视频

Updated: Jun 25, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

通过NMR研究的3型铜蛋白铁酶和基质模拟物p-nitrophenol之间的相互作用.

Armand W J W Tepper1, Luigi Bubacco, Gerard W Canters

  • 1Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.

Journal of the American Chemical Society
|January 13, 2005
PubMed
概括

酶铁酶 (Ty) 与竞争性抑制剂p-nitrophenol (pnp) 相互作用. 这是一种竞争性抑制剂. 磁性核磁共振 (NMR) 显示pnp结合而不会取代关键离子,导致铜中心微妙的结构变化,影响磁性.

科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 结构生物学 结构生物学

背景情况:

  • 铁酶 (Ty) 是一种参与氧化反应的3型铜酶.
  • 了解酶-抑制剂相互作用对于阐明催化机制至关重要.

研究的目的:

  • 调查Streptomyces抗生素铁酶 (Ty) 和其抑制剂p-nitrophenol (pnp) 之间的结合相互作用.
  • 为了确定PNP与铁酶活性部位结合的结构和磁性后果.

主要方法:

  • 超磁核磁共振 (NMR) 光谱学. 超磁核磁共振 (NMR) 光谱学.
  • 对解离常数,磁交换参数和NMR放松参数的分析.

主要成果:

  • p-尼托芬醇 (pnp) 与氧化铁酶 (Ty(met)) 和其化物衍生物结合,具有毫米分子亲和力.
  • pnp结合不会取代桥接化离子,但会诱导3型铜协调几何结构的局部变化.
  • 在PNP结合时,磁交换参数 (-2J) 减小,表明铜离子之间的电子通信发生了变化.
  • 一个结构模型的PNP协调到3型铜中心的衍生.

结论:

更多相关视频

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Published on: November 2, 2018

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)
07:22

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Published on: January 12, 2024

相关实验视频

Last Updated: Jun 25, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Published on: November 2, 2018

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)
07:22

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Published on: January 12, 2024

  • PNP的结合方式为我们提供了对氨酶催化机制的洞察.
  • 这些发现有助于解释相关的甲基醇氧化酶酶中缺少基酶活性.
  • 这项研究促进了对金属酶抑制和结构功能关系的理解.