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Updated: Jun 25, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

通过NMR研究的3型铜蛋白铁酶和基质模拟物p-nitrophenol之间的相互作用

Armand W J W Tepper1, Luigi Bubacco, Gerard W Canters

  • 1Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.

Journal of the American Chemical Society
|January 13, 2005
PubMed
概括

酶铁酶 (Ty) 与竞争性抑制剂p-nitrophenol (pnp) 相互作用. 这是一种竞争性抑制剂. 磁性核磁共振 (NMR) 显示pnp结合而不会取代关键离子,导致铜中心微妙的结构变化,影响磁性.

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The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)07:22

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相关概念视频

¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.