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Updated: Jul 5, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

在GFPmut2染色体中酸诱导的光谱变化的动力学

Stefania Abbruzzetti1, Elena Grandi, Cristiano Viappiani

  • 1Dipartimento di Fisica, Università di Parma, Parco Area delle Scienze 7/A, 43100 Parma, Italy.

Journal of the American Chemical Society
|January 13, 2005
PubMed
概括

我们使用pH跳跃和光谱学研究了GFPmut2中酸诱导的光谱变化. 突变揭示了质子结合和结构重组的动态,突出了His148的存在.

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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