溶于水的 gadofullerenes:向高放松性,对pH响应的MRI对比剂
Eva Tóth1, Robert D Bolskar, Alain Borel
1Laboratoire de Chimie Inorganique et Bioinorganique, Ecole Polytechnique Fédérale de Lausanne, EPFL-BCH, CH-1015 Lausanne, Switzerland. eva.jakabtoth@epfl.ch
Journal of the American Chemical Society
|January 13, 2005
概括
溶于水的 gadofullerene 衍生物显示出作为 pH 响应的MRI 对比剂的潜力. 它们的放松性对MRI至关重要,因聚合而随着pH值变化,使它们适应有针对性的成像.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 医疗成像医学成像
背景情况:
- 目前正在探索内分泌的 gadofullerene 衍生物用于医疗应用.
- 溶于水的 gadofullerene 衍生品提供了作为MRI对比剂的潜力.
- 了解它们的放松性属性是开发先进成像工具的关键.
研究的目的:
- 为了描述水溶性 gadofullerene 衍生物的MRI 对比剂特性:Gd@C(60) (((OH) ((x) 和Gd@C(60) [C(COOH) ((2) ] ((10).
- 为了研究磁场强度和温度对它们的放松性的影响.
- 探索这些化合物的pH依赖性行为,用于潜在的pH响应应用.
主要方法:
- 测量各种温度 (278-335 K) 和磁场 (5 x 10~-4) 到 9.4 T) 的水-质子放松度,以生成核磁共振分散 (NMRD) 配置文件.
- 在不同温度和磁场下分析17O放松速率和化学变化,以确定放松机制.
- 动态光散射 (DLS) 测量以表征pH依赖的聚合.
主要成果:
- 在高磁场 (30-60 MHz) 中,Gd@C(60) (((OH) ((x) 和Gd@C(60) ((C(COOH) ((2)))))) 均表现出放松度最大值,最大的放松度分别为38.5 mM(-1) s(-1) 和10.4 mM(-1) s(-1) 在299 K.
- 确定了外球放松机制,旋转运动缓慢,旋转相关时间长 (tau(R) ((298) = 2.6 ns).
- 随着pH值 (3-12) 的下降,由于pH值依赖的聚合,质子放松率显著增加,这得到了DLS的证实.
结论:
- 描述的 gadofullerene 衍生物具有显著的放松性,Gd@C(60)(OH)(x) 显示较高的值.
- 观察到的依赖pH的聚合和随后的放松性变化使这些化合物成为响应pH的MRI对比剂的有希望的候选者.
- 进一步开发可能会导致针对特定生理环境做出反应的有针对性的诊断工具.
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