染色体Chd1链接了基因组H3甲基化与SAGA和SLIK依赖的乙化
Marilyn G Pray-Grant1, Jeremy A Daniel, David Schieltz
1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA.
Nature
|January 14, 2005
概括
研究人员发现Chd1 (chromo-ATPase/helicase-DNA结合域1) 与甲基化组素H3结合. 这种相互作用增强了SAGA和SLIK基因组酸转移酶复合物的活性,影响了基因调节.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
背景情况:
- 基因组的翻译后修改调节了核过程,如基因调节和DNA修复.
- 希斯乙转移酶 (HATs) 是转录激活中的关键酶.
- 酵母SAGA和SLIK是保存的HAT复合体,涉及素乙化和二氧化化.
研究的目的:
- 为了确定SAGA和SLIK复合物的新组件.
- 为了研究染色体重塑蛋白在基因素修饰途径中的作用.
- 描述Chd1和基因组修饰之间的相互作用.
主要方法:
- 染色体免疫沉,然后进行质谱测量以确定SAGA/SLIK组件.
- 在体外结合试验中,使用重组蛋白和纯化的素进行了结合试验.
- 在体内研究使用酵母遗传学和显微镜来评估复杂的功能.
主要成果:
- 染色体重塑蛋白Chd1 (染色ATPase/酶-DNA结合域1) 被确定为SAGA和SLIK的一个组成部分.
- Chd1的一个染色体特别识别了在lysine 4 (H3K4me) 处的甲基化组素H3.
- SLIK复合体表现出甲基化基质的增强乙化,这取决于Chd1的甲基结合染色体.
结论:
- 这项研究确定了第一个识别H3K4me.me的染色体.
- 基因组甲基化标记和乙化之间的关键联系是chd1.
- 建议使用一种具有相似识别特性的染色体蛋白的潜在子家族.
相关概念视频
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:25Spreading of Chromatin Modifications
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Writers
The writer is an enzyme that can...
03:17Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...