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相关概念视频

Internal Receptors01:31

Internal Receptors

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Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
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Short-distance Transport of Resources02:12

Short-distance Transport of Resources

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Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
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Types of Receptors: Internal Receptors01:07

Types of Receptors: Internal Receptors

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Many cellular signals are hydrophilic and cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind intracellular receptors that reside within the cell cytoplasm or nucleus. Many mammalian steroid hormones and nitric oxide (NO) gas use this cell signaling mechanism.
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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MAPK Signaling Cascades01:07

MAPK Signaling Cascades

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Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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相关实验视频

Updated: May 4, 2026

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
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类固醇与植物受体激酶BRI1的细胞外域的结合.

Toshinori Kinoshita1, Ana Caño-Delgado, Hideharu Seto

  • 1Howard Hughes Medical Institute and Plant Biology Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, California 92037, USA.

Nature
|January 15, 2005
PubMed
概括

植物类固醇或类固醇直接与BRI1受体激酶结合. 这种结合发生在BRI1细胞外域的特定的94氨基酸区域内,识别出一种新的类固醇激素结合部位.

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科学领域:

  • 植物生物学 植物生物学
  • 分子信号传递是分子信号传递.
  • 生物化学 生物化学

背景情况:

  • 类固醇在动物和植物中起到关键的信号分子作用.
  • 在植物中,类固醇是关键的信号分子,而BRI1是一个关键的血膜受体激酶,参与了它们的感知.
  • 动物类固醇的识别主要涉及核受体.

研究的目的:

  • 提供第一个直接证据,证明活跃的布拉辛类固醇与BRI1受体激酶结合.
  • 为了确定BRI1中负责布拉辛固醇结合的特定域.

主要方法:

  • 在结合性研究中使用了生物标记的光亲和卡斯塔斯特 (BPCS).
  • 使用的3H标记布拉西诺化物和重组BRI1碎片.
  • 进行了结合测试以确定最小的结合域.

主要成果:

  • 证明了类固醇与BRI1.1的直接结合.
  • 确定了LRR21和LRR22之间的70个氨基酸岛域 (ID),以及旁边的碳酸终端LRR (ID-LRR22),作为最小结合域.
  • ID-LRR22区域包括BRI1细胞外域中的94个氨基酸,是布拉辛固醇的直接结合部位.

结论:

  • 铜类固醇直接与BRI1受体激酶的细胞外域内的特定区域 (ID-LRR22) 结合.
  • 这项研究定义了植物中类固醇激素的新型结合域.
  • 阐明了植物布拉西诺固醇信号传递中的关键分子相互作用.