在试验室中对mRNA前拼接的阳性控制
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
概括
变压器 (tra) 和变压器-2 (tra-2) 基因控制Drosophila中的性别特异拼接. 它们在外子上促进蛋白质复合体,促进对女性发育的拼接部位识别.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
背景情况:
- 替代拼接调节基因表达,对性差异化至关重要.
- 双性 (dsx) 基因的替代拼接是Drosophila melanogaster.性发育的关键.
- 变压器 (tra) 和变压器-2 (tra-2) 蛋白质是已知的 dsx 拼接的调节者.
研究的目的:
- 为了研究体外机制,变压器 (tra) 和变压器-2 (tra-2) 激活双性 (dsx) 前mRNA的女性特异性3'拼接部位.
- 确定参与这种性别特异性替代拼接事件的调节元素和蛋白质相互作用.
主要方法:
- 使用了体外剪接系统来研究dsx前mRNA处理.
- 用重组变压器 (Tra) 和变压器-2 (Tra-2) 蛋白来激活女性特有的3'拼接部位.
- 进行了电泳运动转移试验 (EMSAs),以检测蛋白质与调节性外子序列的结合.
主要成果:
- 一个在体内必不可少的外显子序列被发现是必要的,并且足以在体内激活女性特异的3'拼接位.
- 再组合的Tra和Tra-2蛋白可以激活体外系统中的拼接部位.
- 证明了包括Tra和Tra-2在内的核蛋白与调节性外子序列的特定结合.
结论:
- 特拉和特拉-2可能通过促进多蛋白质复合物的组装在dsx前mRNA的特定表子序列上起作用.
- 这种多蛋白质复合体可能会增强相邻的雌性特异性3'拼接部位被拼接机器识别.
- 这些发现为Drosophila的性别特定替代拼接的调节提供了机理性的洞察力.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...


